منابع مشابه
Isobutanol tolerance in Ralstoniaeutropha
Background Ralstonia eutropha is bacterium known to naturally produce polyhydroxybutyrate (PHB) as carbon storage during nutrient starvation. Previously studies [1] showed that it is possible through the incorporation of an engineered biosynthetic pathway, to redirect carbon flux from PHB to the production of Isobutanol (IBT), a biofuel largely studied to replace the current fossil fuels in exi...
متن کاملEvolution, genomic analysis, and reconstruction of isobutanol tolerance in Escherichia coli
Escherichia coli has been engineered to produce isobutanol, with titers reaching greater than the toxicity level. However, the specific effects of isobutanol on the cell have never been fully understood. Here, we aim to identify genotype-phenotype relationships in isobutanol response. An isobutanol-tolerant mutant was isolated with serial transfers. Using whole-genome sequencing followed by gen...
متن کاملEvolution combined with genomic study elucidates genetic bases of isobutanol tolerance in Escherichia coli
BACKGROUND Isobutanol is a promising next-generation biofuel with demonstrated high yield microbial production, but the toxicity of this molecule reduces fermentation volumetric productivity and final titer. Organic solvent tolerance is a complex, multigenic phenotype that has been recalcitrant to rational engineering approaches. We apply experimental evolution followed by genome resequencing a...
متن کاملIsobutanol production from cellobionic acid in Escherichia coli
BACKGROUND Liquid fuels needed for the global transportation industry can be produced from sugars derived from plant-based lignocellulosics. Lignocellulosics contain a range of sugars, only some of which (such as cellulose) have been shown to be utilizable by microorganisms capable of producing biofuels. Cellobionic acid makes up a small but significant portion of lignocellulosic degradation pr...
متن کاملIsobutanol production at elevated temperatures in thermophilic Geobacillus thermoglucosidasius.
The potential advantages of biological production of chemicals or fuels from biomass at high temperatures include reduced enzyme loading for cellulose degradation, decreased chance of contamination, and lower product separation cost. In general, high temperature production of compounds that are not native to the thermophilic hosts is limited by enzyme stability and the lack of suitable expressi...
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ژورنال
عنوان ژورنال: BMC Proceedings
سال: 2014
ISSN: 1753-6561
DOI: 10.1186/1753-6561-8-s4-p232